Author information
1
Department of Medicine, NAFLD Research Center, La Jolla, California.
2
Université Lyon 1, Hospices Civils de Lyon, Lyon, France.
3
Department of Radiology, University of California at San Diego, La Jolla, California.
4
Liver Imaging Group, Department of Radiology, University of California at San Diego, La Jolla, California.
5
Human Biology, J. Craig Venter Institute, La Jolla, California.
6
Division of Gastroenterology, Department of Medicine, La Jolla, California.
7
Division of Epidemiology, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, California.
Abstract
Previous studies have shown that gut-microbiome is associated with nonalcoholic fatty liver disease (NAFLD). We aimed to examine if serum metabolites especially those derived from the gut-microbiome have a shared gene-effect with hepatic steatosis and fibrosis. This is a cross-sectional analysis of a prospective discovery cohort including 156 well-characterized Twins and Families with untargeted metabolome profiling assessment. Hepatic steatosis was assessed using magnetic-resonance-imaging proton-density-fat-fraction (MRI-PDFF) and fibrosis using MR-elastography (MRE). A twin additive genetics and unique environment effects (AE) model was used to estimate the shared gene-effect between metabolites and hepatic steatosis and fibrosis. The findings were validated in an independent prospective validation cohort of 156 participants with biopsy-proven NAFLDincluding shotgun metagenomics sequencing assessment in a subgroup of the cohort. In the discovery cohort, 56 metabolites including 6 microbial metabolites had a significant shared gene-effect with both hepatic steatosis and fibrosis after adjustment for age, sex and ethnicity. In the validation cohort 6 metabolites were associated with advanced fibrosis. Among them, only one microbial metabolite, 3-(4-hydroxyphenyl)lactate, remained consistent and statistically significantly associated with liver fibrosis in the discovery and validation cohort (fold-change of higher-MRE versus lower-MRE:1.78,p<0.001 and of advanced versus no advanced fibrosis:1.26,p=0.037, respectively). The share genetic determination of 3-(4-hydroxyphenyl)lactate with hepatic steatosis was RG :0.57,95%CI:0.27-0.80,p<0.001 and with fibrosis was RG :0.54,95%CI:0.036-1,p=0.036. Pathway reconstruction linked 3-(4-hydroxyphenyl)lactate to several human gut-microbiome species. In the validation cohort, 3-(4-hydroxyphenyl)lactate was significantly correlated with the abundance of several gut-microbiome species, belonging only to Firmicutes, Bacteroidetes and Proteobacteria phyla, previously reported as associated with advanced fibrosis.
CONCLUSION:
This proof of concept study provides evidence of a link between the gut-microbiome and 3-(4-hydroxyphenyl)lactate that shares gene-effect with hepatic steatosis and fibrosis.