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Abstract Details
Serum ?1-AT Levels and Molecular Analysis in Breast Cancer: An Experimental and Computational Study.
Ávalos-Navarro, Guadalupe (G);Bautista-Herrera, Luis A (LA);Garibaldi-Ríos, Asbiel Felipe (AF);Ramírez-Patiño, Ramiro (R);Gutiérrez-García, Marisol (M);Briseño-Álvarez, Perla (P);Jave-Suárez, Luis Felipe (LF);Reyes-Uribe, Emmanuel (E);Gallegos-Arreola, Martha Patricia (MP);
BACKGROUND/OBJECTIVES: Breast cancer (BC) is a heterogeneous disease with multifactorial origins, including environmental, genetic, and immunological factors. Inflammatory cytokines, such as alpha 1 antitrypsin (α1-AT), are increased in BC and affect physiological and pathological conditions. This study aimed to evaluate the serum levels of α1-AT and perform a computational analysis of in BC, as well as their association with molecular subtypes and clinical features.
METHODS: For the experimental analysis, we evaluated 255 women with BC and 53 healthy women (HW) in a cross-sectional study. Molecular subtypes were identified by immunohistochemistry and TNM was used for clinical staging. Soluble levels of α1-AT were quantified by ELISA. Computational analysis of expression was performed using GEPIA and cBioPortal.
RESULTS: α1-AT was increased in BC women versus HW (75.8 ng/mL vs. 532.2 ng/mL). Luminal A had higher concentration (547.5 ng/mL) than Triple Negative (TN) (484.1 ng/mL), but the levels were not associated with clinical stage. The computational analysis showed that is overexpressed in BC with differential expression among subtypes; its overexpression is associated with a better prognosis, longer disease-free survival, and overall survival.
CONCLUSIONS: α1-AT levels are increased in women with BC women compared to HW. The Luminal A subtype shows higher soluble protein levels than the TN one. Furthermore, mRNA overexpression in BC is linked to a protective effect.